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Scientia Silvae Sinicae ›› 2015, Vol. 51 ›› Issue (5): 159-164.doi: 10.11707/j.1001-7488.20150519

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Isolation and Identification of a Mycorrhiza Helper Bacteria(MHB)—Brevibacillus reuszeri on Pinus massoniana Roots

Li Qian, Wu Xiaoqin, Ye Jianren   

  1. Foretry College,Nanjing Forestry University Southern Modern Forestry Cooperative Innovation Center Nanjing 210037
  • Received:2013-12-23 Revised:2015-03-03 Online:2015-05-25 Published:2015-06-11

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Abstract:

【Objective】 To investigate the interaction between ectomycorrhizal fungi and mycorrhiza helper bacteria, we screened 74 bacterial strains isolated from the rhizosphere soil of Pinus massoniana that bore the fruiting body of Pisolithus tinctorius.【Method】The dry-plate confrontation assay was used to screen the bacterial strains, and one strain of MHB was obtained and defined as MPt17 which can efficiently promote the growth of Pisolithus tinctorius (Pt2) and Xerocamus chrysenteron (Xc). 【Result】The result showed that obtained MPt17 significantly promoted in vitro hyphal growth of Pt2 and Xc with an increasing rate of 48.6% and 25.0%. The extracellular metabolites of Mpt17 were able to also obviously promote the increment rate of Pt2 and Xc hypha by 129.6% and 29.5%, and consistently influence on Pt2 and Xc biomass increment with an increasing rate of 124.2% and 34.2%. Compared with the blank control, the pine seedlings co-inoculated with Pt2 and MPt17 strain, co-inoculated with Xc and MPt17 strain, Pt2 alone, Xc alone, and MPt17 alone all significantly increased their mean height with increasing rates of 78.0%, 68.2%, 32.7%, 39.2% and 53.4%, and their diameter with increasing rates of 46.3%, 57.5%, 17.5%, 23.8% and 25.0%. According to our results, Mpt17 was not only a Mycorrhiza Helper Bacterium but also a plant growth-promoting rhizobacterium. The MHB stain was identified based on an analysis of morphologiacal characters, the BIOLOG system and 16SrRNA gene sequences homology. MPt17 was identified as Brevibacillus reuszeri, and the 16SrRNA sequences have been registered at GenBank database under the accession number of KF891383.【Conclusion】In this experiment the pine seedlings co-inoculated with Pt2 and MPt17 strain, and co-inoculated with Xc and MPt17 strain significantly improved the growth of pine seedlings, and MPt17 alone also improved the growth of seedlings to a certain extent. This study method with the interactions of ectomycorrhizal fungi, Mycorrhiza Helper Bacteriun and plant has important reference implications for similar studies.

Key words: Pinus massoniana, ectomycorrhizal fungi, mycorrhiza helper bacteria, Brevibacillus reuszeri

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