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Scientia Silvae Sinicae ›› 2003, Vol. 39 ›› Issue (3): 48-52.doi: 10.11707/j.1001-7488.20030308

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ESTIMATION OF THE GENETIC HETEROZYGOSITY OF POPULUS TOMENTOSA AND ITS HYBRID P.TOMENTOSA×P. BOLLEANA USING AFLP TECHNIQUE

Zhang Deqiang1,Zhang Zhiyi2,Song Wan4,Yang Kai3   

  1. Institute of Populus tomentosa, BFU Beijing 100083;Institute of Crop Germplasm, CAA Beijing 100081
  • Received:2002-09-29 Revised:1900-01-01 Online:2003-05-25 Published:2003-05-25

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Abstract:

The average heterozygosity of male clone “LM 50” (Populus tomentosa) and its hybrid female clone “TB01”(P.tomentosa×P.bolleana) was estimated, using AFLP (Amplified Fragment Length Polymorphisms) technique, based on random samples with 120 progeny. The hybrids were derived from an interspecific backcross between the female hybrid clone “TB01” and the male clone “LM50”. The result showed that the average heterozygosity was 18.71% with a range of 9.10% ～ 30.19% for male clone “LM50” (P. tomentosa), and 8.47% varied from 1.39% to 20.31% for female clone “TB01” (P.tomentosa×P.bolleana), respectively, using 30 different AFLP primer combinations. Therefore, the average heterozygosity of clone “LM50” was 2.21 times larger than the one of clone “TB01”, based on the segregation population.

Key words: Populus tomentosa, Populus tomentosa×, P. bolleana, Genetic heterozygosity, AFLP technique