Abstract:

This study intended to establish a culture system that would induce somatic embryogenesis of Acanthopanaxsenticosus. Various plant growth regulators and their ratio were investigated with different explants of A.senticosus. The results showed that the leaf was the best organ to induce callus, followed by the stem, and the root. April and May were the best period to collect explants for the induction. The best induction medium was MS supplemented with 1.0 mg·L^-12,4D and 0.5 mg\5L^-1 6 BA for callus of A. senticosus. MS alone was the best medium for growth and differentiation. The observed anatomy displayed that the ex vivo embryogenesis process was the same as the zygotic embryo development process of A. senticosus.

Key words: Acanthopanax senticosus, somatic embryo, plant regeneration