Abstract:

Real-time fluorescent quantitative PCR was used to datect DREB genes' expression levels in leaves of Broussonetia papyrifera seedlings at 0, 6, 12 and 24 h after treatments with 100, 200 and 300 mmol·L^-1 NaCl. The results showed that BpDREB1 and BpDREB2 genes were induced by 100 and 200 mmol·L^-1 NaCl to increase their expression levels. But the levels decreased at later stage by the salt stress. Treatment with 300 mmol·L^-1 NaCl caused reduced expression levels of the two genes. The experiment verified that the transcriptional expression of DREB genes in B. papyrifera was able to be induced by salt stress, which might be involved in signalling pathways in response to the salt stress. But the genes were prohibited by higher salt concentration or longer duration of NaCl stress.

Key words: real-time fluorescent quantitative PCR, Broussonetia papyrifera, DREB transcription factor, C_T value, salt stress