Abstract:

In this work the supercritical fluid extraction with carbon dioxide was applied to extract roots of Stellera chamaejasme. The extracts were evaluated through the antifungal activity against Monilinia fructicola. Furthermore, the active compounds from the extracts were separated by macroporous resin and analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that the extracts had strong antifungal activity against M. fructicola with inhibition ratio of 88.71% at 2 000 μg·mL^-1, minimum inhibitory concentration (MIC) of 250 μg·mL^-1, and minimum fungicidal concentration (MFC) of 2 000 μg·mL^-1. After separated with macroporous resin chromatography, fourteen fractions were collected from the extracts. Fraction 4, 7, and 12 in a concentration of 2 000 μg·mL^-1 presented highly antifungal activity against M. fructicola, with inhibition ratio of 83.97%, 80.23%, and 75.69%, respectively. The GC-MS analysis revealed that the main active compounds from fraction 4, 7, and 12 included hexanedioic acid;bis(2-ethylhexyl) ester;β-sitosterol;7-methyl-Z-tetradecen-1-ol acetate9;9-hexadecenoic acid,hexadecyl ester,(Z);1,2-benzenedicarboxylic acid,diisooctyl ester;(3β,24Z) ,stigmasta-5,24(28)-dien-3-ol;stigmastan-3,5-diene, and squalene.

Key words: Stellera chamaejasme, SC-CO₂ extraction, antifungal activity, Monilinia fructicola