Abstract:

Effects of GA₃, IBA as well as different cultivating temperature on scale cutting propagation were studied in Lilium Asiatic hybrid 'Elite’. The results indicated that low concentration of IBA(IBA-80 mg ·L^-1-1 h)was better for improving propagation coefficient and produced higher uniformity of bulblets. IBA treatment promoted enlargement of bulblets within a short period. However, when the culture duration was long enough, GA₃ was better for producing larger bulblets. With low temperature (5 ℃) pretreatment IBA accelerated the degradation of starch and the utilization of soluble sugar in the mother scales, further improved scale propagation coefficient and average grade as well as single bulblet diameter. But GA₃ with the low temperature pretreatment inhibited the starch degradation in the mother scales and the utilization of soluble sugar in the bulblets, resulting in the bulblets grew slowly. Under the condition of this experiment, IBA-100 mg ·L^-1-1 h with low temperature both 5 ℃ pretreatment was the best treatment for improving the propagation cofficient and uniformity of bulblets, and GA₃-100 mg ·L^-1-2 h with even 25 ℃ culture was most conductive to expansion of bulblets when cultured in a constant temperature of 25 ℃, which was the best treatment to obtain the excellent individuals.

Key words: lily, scale cutting, growth regulator, alternating temperature, starch degradation