Abstract:

The pine wood nematode is a plant parasitic nematode that causes devastating pine-wilt disease. Previous studies have indicated a high degree of genetic heterogeneity between isolates and within isolate. To increase homozygosity, the pine wood nematode was inbred by sibling matings at each generation for 20 generations. Total of 80 inbred lines which from 3 parents of virulent isolate AMA3 were created. The virulence potential of inbred lines in different generation was estimated by inoculation test using susceptible Pinus thunbergii seedlings and then by comparing the resulting host mortality with the original nematode isolate. As a result, the successive inbreeding did not significantly changed the virulence, and inbred lines in 6,10,15 and 20 generation were the same in virulence compared to the original isolates.

Key words: Bursaphelenchus xylophilus, full-sibling, inbreeding, virulence