利用ISSR DNA标记鉴定主要银杏栽培品种

doi:10.11707/j.1001-7488.20050134

Scientia Silvae Sinicae ›› 2005, Vol. 41 ›› Issue (1): 202-204.doi: 10.11707/j.1001-7488.20050134

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Identification on the Main Cultivated Varieties of Ginkgo biloba Using ISSR DNA Marker

Shen Yongbao¹,Shi Jisen¹,Zhao Hongliang²

1.Nanjing Forestry University Southern Tree Seed Inspection Center Nanjing 210037; 2.Pizhou Forest Administration, Jiangsu Province Pizhou221400

Received:2003-12-24 Revised:1900-01-01 Online:2005-01-25 Published:2005-01-25

Abstract

Abstract:

Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46-550,ISSR46-670,ISSR46-740,ISSR46-1000,ISSR46-1159,ISSR46-1359,ISSR46-1600,ISSR44-520,ISSR44-580,ISSR44-660,ISSR44-1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker.

Key words: Ginkgo biloba, ISSR marker, identification

Shen Yongbao;Shi Jisen;Zhao Hongliang. Identification on the Main Cultivated Varieties of Ginkgo biloba Using ISSR DNA Marker[J]. Scientia Silvae Sinicae, 2005, 41(1): 202-204.

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Identification on the Main Cultivated Varieties of Ginkgo biloba Using ISSR DNA Marker

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