杨树皮储藏蛋白基因启动子的克隆和功能研究*

Abstract

Abstract:

Bark storage protein(BSP)of poplar, anitrogen reserve similar to seed storage protein, plays an important role in the nitrogen metabolism of deciduous trees.They present in large quantities in the phloem parenchyma cells in winter.In order to learn the expression pattern of BSP gene promoter in transgenic plants, and to explore its potential application in plant genetic engineering for phloem-specific expression of interested genes.BSP gene promoter fragment was amplified by PCR method from Populus deltoides genome DNA.Intermediate vector was constructed by inserting the promoter sequence upstream of GUS gene in pBI121, resulting in the replacement of the original CaMV 35S promoter by the BSP promoter. Regenerated tobacco plants obtained by transformation were analyzed by PCR to select the putative transgenic plans.Histochemical localization of GUS activity indicated that BSP promoter could confer a phloemspecific expression of GUS gene.

Key words: Populus deltoides, Phloem-specific promoter, GUS gene, Transgenic tobacco plants

Hao Jiang,Hongmin Qin,Yingchuan Tian,W.Gabriel Dean. CLONINGOF A PROMOTER FRAGMENTOF BARK STORAGE PROTEIN GENE FROM POPULUS DELTOIDES AND ITS FUNCTION IN TRANSGENIC TOBACCO PLANTS[J]. Scientia Silvae Sinicae, 1999, 35(5): 46-50.

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CLONINGOF A PROMOTER FRAGMENTOF BARK STORAGE PROTEIN GENE FROM POPULUS DELTOIDES AND ITS FUNCTION IN TRANSGENIC TOBACCO PLANTS

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