重大害虫长林小蠹入侵我国的首次发现与侵染特征

doi:10.11707/j.1001-7488.20210513

Abstract

Abstract:

Objective: This study aims to clarify the invasion, colonization, and severe damage of the major international forest quarantine pest Hylurgus ligniperda in China. The morphological characteristics, molecular identification, and damage characteristics were studied, so as to provide a theoretical basis for the monitoring and control of this pest. Method: The morphological characteristics of the larvae, pupae, and adults H. ligniperda collected from the roots of Pinus thunbergii in Muping District, Yantai City, Shandong Province were observed, photographed, and compared. The genomic DNA was extracted, and the sequences of the large ribosomal subunit 28S and mitochondrial cytochrome oxidase subunit Ⅰ(COⅠ) were amplified and sequenced using general primers of the subfamily. The sequences were compared with the existing sequences in the NCBI database. The damage characteristics of H. ligniperda were preliminarily investigated. Result: The morphological characteristics of the adults were completely consistent with the description of H. ligniperda. The phylogenetic tree (ML tree) based on the 28S gene sequences showed that the samples from Yantai Shandong, Xiamen Port intercepted samples from Australian Radiata Pine logs, the German samples, and the American samples were clustered into one branch with 100% confidence. The Yantai Shangdong samples shared the same haplotype with the H. ligniperda samples intercepted from New Zealand Radiata Pine logs based on COⅠ sequence, indicating that the invasive alien species H. ligniperda has been established in China. This insect seriously damages roots of the host trees. Conclusion: It is the first time to find the colonization and serious damage of the major invasive pest, H. ligniperda, in China. The main morphological characteristics have been identified, and the infestation characteristics have been preliminarily clarified. The results of gene sequence analysis provide a basis for developing rapid identification technology and traceability analysis. This study would provide a theoretical basis and technical support for its detection and monitoring.

Key words: Hylurgus ligniperda, morphological characteristics, molecular identification, damaging characteristics

CLC Number:

Lili Ren,Jing Tao,Haiwei Wu,Shixiang Zong,Chuanzhen Wang,De Hua,Juan Shi,Yizhou Liu,Youqing Luo. The First Discovery and Infective Characteristics of A Major Invasive Pest Hylurgus ligniperda (Coleoptera: Scolytidae) in China[J]. Scientia Silvae Sinicae, 2021, 57(5): 140-150.

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序号 No.	基因 Gene	引物名称 Primers	引物序列 Sequences	退火温度 Annealing T/℃	参考文献 References
1	COⅠ	S1718	5'-GGAGGATTTGGAAATTGATTAGTTCC-3'	46	Jordal et al., 2011
2		A2411	5'-GCTAATCATCTAAAAACTTTAATTCCWGTWG-3'	46
3	28S	S3690	5'-GAGAGTTMAASAGTACGTGAAAC-3'	55
4		A4394	5'-TCGGAAGGAACCAGCTACTA-3'	55

NCBI登录号 NCBI accession No.	物种名称 Species	采集地 Location
JX263769	长林小蠹 Hylurgus ligniperda	新西兰奥克兰 Auckland, New Zealand
MH005064	长林小蠹 Hylurgus ligniperda	厦门口岸截获来自于澳大利亚辐射松 Xiamen port intercepted from the Australian Radiata Pine
KJ531125	长林小蠹 Hylurgus ligniperda	德国Germany
JX263770	米林小蠹 Hylurgus micklitzi	西班牙Spain
HQ883927	Hylastes opacus	瑞典Sweden
KJ531123	Hylastes attenuates	德国Germany
AF308364	Hylurgops rugipennis	美国USA
JX263728	Hylurgops glabratus	挪威Norway
AF308365	Hylesinus varius	美国USA
JX263725	Hylesinus toranio	英格兰England
HQ883592	纵坑切梢小蠹 Tomicus piniperda	挪威Norway
AF308386	黑脂大小蠹 Dendroctonus terebrans	美国USA
HQ883591	云杉大小蠹 Dendroctonus micans	爱沙尼亚Estonia
AF308385	山松大小蠹 Dendroctonus ponderosae	美国USA
AF308380	间大小蠹 Dendroctonus adjunctus	美国USA
FJ867678	黄杉大小蠹 Dendroctonus pseudotsugae	美国USA
MH997403	南方松大小蠹 Dendroctonus frontalis	墨西哥Mexico
JX263669	Bothrosternus foveatus	哥斯达黎加Costa Rica

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The First Discovery and Infective Characteristics of A Major Invasive Pest Hylurgus ligniperda (Coleoptera: Scolytidae) in China

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