Abstract: [Objective] This research was aimed to study the technical parameters for efficient production of biochemical fulvic acid (BFA) by fermentation of Molasses Alcohol Wastewater and provide a theoretical basis for the efficient production of BFA. [Method] Single-factor and orthogonal experiment were applied to test effects of inoculation amount and inoculation ratio of Geotrichhum candidum, Aspergillus niger and Bacillus pumilus, culture medium composition and fermentation period etc. on production of BFA. The aluminum sulfate flocculation gravimetric method was used for the determination of BFA. This study was conducted to identify fermentation conditions and culture medium of efficient production of BFA in fermentation broth. [Result] In the inoculation amount of 5%, the BFA content produced by different proportion of Geotrichhum candidum, Aspergillus niger and Bacillus pumilus was 221.2-236.5 g·L^-1, and the best proportion was 1:1:2. When the concentration of adding glucose was less than 15 g·L^-1, the of BFA content increased with the increase of glucose, but when it was more than 5%, the increase of BFA production was not significant. When the urea was less than 1.5%, the content of BFA increased with the increase of urea. [Conclusion] As the inoculation ratio of Geotrichhum candidum, Aspergillus niger and Bacillus pumilus was 1:1:2, inoculation amount was 6%, the content of glucose, urea and ammonium sulfate were 50.0, 15.0 and 2.0 g·L^-1, and the fermentation time was 72 h, the production of BFA was the highest, 278.9 g·L^-1. The effects of several factors on the production of BFA were decreased in the following order of:the concentration of glucose, the fermentation period, the amount of urea, the concentration of inoculation, the amount of ammonium sulfate.

Key words: Geotrichhum candidum, Aspergillus niger, Bacillus pumilus, coinstantaneous fermentation