中国沙棘种子的水引发技术及其抗性生理效应

doi:10.11707/j.1001-7488.20141205

Abstract

Abstract:

Optimum conditions for seed priming of sea buckthorn (Hippophae rhamnoides subsp. sinensis) were determined by investigating the effects of different temperatures and water absorption rates on germination of sea buckthorn. Furthermore, the physiology effect of seed priming was investigated by evaluating the vigor, morphological indexes of seedlings under drought and salt stress conditions. Results showed that hydropriming condition was optimum at 10 ℃, providing water in batches to the seed in dark for seven days. The total volume of water used for hydropriming was 55% of the original mass of seeds. The 23%, 23%, 25%, 14% and 15% of the total water volume were added in 0, 3, 6, 12, 21 h of hydropriming, respectively. The hydropriming effects of sea buckthorn seeds were evaluated by measuring the content of soluble sugar, MDA and proline in seeds from 4 different provinces (Shaanxi, Ningxia, Gansu, Inner Mongolia). Hydropriming seeds of sea buckthorn significantly improved seed germination rate and germination index. The hydropriming also reduced mean germination time and increased seedling height, root length, fresh weight, and enhanced critical germination conditions under drought and salt stress conditions as compared to control. Comprehensive analysis indicated that hydropriming could control seed imbibitions and prevent cell membrane structure from being destroyed, thereby preventing the leakage of soluble substances. This action significantly improved seed soluble sugar content and hence provided a guarantee for germination. It also decreased MDA content and enhanced the function of seed antioxidant system. The hydropriming significantly increased proline content, thereby enhancing seed resilience. Hydropriming technology is inexpensive and it may be an effective way of enhancing seed vigor and seedling resistance.

Key words: hydropriming, sea buckthorn, seed germination, seed vigor, seedling resistance

CLC Number:

S718.43

Sun Miao, Yang Zhouting, Zhang Cunli, Wei Anzhi, Shi Wei. Hydro-Priming Technique and Its Resistance Physiology Effect for Sea Buckthorn Seed[J]. Scientia Silvae Sinicae, 2014, 50(12): 32-39.

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Hydro-Priming Technique and Its Resistance Physiology Effect for Sea Buckthorn Seed

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