一色齿毛菌产MnP的条件优化及对染料的脱色

doi:10.11707/j.1001-7488.20140717

Abstract

Abstract:

Manganese peroxidase(MnP) is secreted by white-rot fungi, it can degrade lignin and various xenobiotics efficiently. Cerrena unicolor was firstly cultured under initial conditions and the curve of its MnP activity was gained. Then, 5 kinds of carbon sources and 5 kinds of nitrogen sources were selected, and two orthogonal tests were conducted to optimize medium compositions and culture conditions of C. unicolor producing MnP. Optimized extracellular MnP solutions were determined to decolour 5 kinds of dyes. Results showed that the most optimal culture parameters for MnP production was fructose 10 g·L^-1, ammonium nitrate 0.2 g·L^-1, Mn²⁺2.67 mmol·L^-1,pH 5.5, 70 mL culture solution in 250 mL flask at 150 r·min^-1 shaking condition, 9 tablets of Ø=8 mm inocula, and temperature 34℃. The highest MnP activity reached 95.47 U·L^-1after 7 d culture, which was 5.2 times than that produced at initial conditions. Decolorization test showed that optimized extracellular MnP solutions of C. unicolor had effectively decoloring ability to 5 kinds of dyes belonging to 3 kinds of group. The decolorization rate of the 5 kinds of dye all reached maximum after 8 d. On 8 days of incubation, reactive black was almost completely decolorized, the decolorization percent was as high as 98.4%, and at that time the decolorization percents to congo redr, reactive red, crystal violet, and neutral red were also as high as 72.3%-89.4%.

Key words: Cerrena unicolor, manganese peroxidase(MnP), optimized culture, dye decolorization, reactive black

CLC Number:

Q936

Yu Cun, Chi Yujie. Optimization of Culture Conditions of MnP Produced by Cerrena unicolor and Its Decolorization to 5 Kinds of Dyes[J]. Scientia Silvae Sinicae, 2014, 50(7): 121-127.

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Optimization of Culture Conditions of MnP Produced by Cerrena unicolor and Its Decolorization to 5 Kinds of Dyes

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