杨树苗期抗寒性综合评价体系的构建

doi:10.11707/j.1001-7488.20140707

Abstract

Abstract:

The germination rate of annual branches of 11 poplar (Populus) clones were used as a judging indicator to investigate the cold resistance after cold hardiness treatment with low-temperature and freezing-thawing at-40℃ for 24 hours and then transferred to 20℃ for 24 hours repeatedly four times. Five parameters, such as relative electric conductivity, relative water content, water saturation deficit, MDA and soluble sugar, were selected by using correlation analysis for studying physiological and biochemical substances, physical characters, growth traits and phenological phases etc. The parameters were highly significantly or significantly correlated to the hardiness. The five parameters were further converted to integrated index by principle component analysis, with the combination of their subordinate functions to establish an assessment system. Integrated index value of cold resistance of each variety was obtained by measuring the weight and its function value. According to composite index, the sequence of cold resistance from strong to weak was P.×xiaohei, P. ×xiaozhuanica ‘Poplar’, P. deltoides ×(P. nigra var. thevestina ×P. nigra), P. simonii, P. ×canadensis ‘N3016’, P. ×canadensis ‘Guariento’, P. ×canadensis ‘Sacran-79’, P. ×deltoides ‘Liaoyu3’, P. ×euramericana ‘97’, P. ×euramericana ‘Zhongliao1’, P. ×euramericana ‘L35’. The result was consistent with the performance in cold resistance of the clones in field. These clones of poplars could be divided into four groups by cluster analysis. The first group, the first 4 clones in the resistance sequence, had the strongest cold resistance. The second group, including the 5-7^th in the sequence, had medium cold resistance. The third group, including the 8-10^th in the sequence, had relative weak resistance. The last one in the sequence was the worst. The evaluation system provided a technical support to judge the ability of cold resistance of seedlings of different clones, and would be beneficial to others such as breeding for cold resistance, varieties classification.

Key words: poplar, cold resistance, freezing and thawing, integrated assessment, physiological and biochemical indexes

CLC Number:

S718.43

Li Xiaoyu, Yang Chengchao, Peng Jiandong, Yang Zhiyan, Zhang Yan. Establishment of an Integrated Assessment System on Cold Resistance of Poplars at the Seedling Stage[J]. Scientia Silvae Sinicae, 2014, 50(7): 44-51.

References

高京草,王慧霞,李西选.2010.可溶性蛋白、丙二醛含量与枣树枝条抗寒性的关系研究.北方园艺,(23):18-20.
(Gao J C, Wang H X, Li X X. 2010. Relationship between soluble protein,MDA,and jujube tree cold hardiness. Northern Horticulture, (23):18-20. [in Chinese] )
李国华,岳海,庞育文,等.2009.西双版纳地区引种的澳洲坚果抗寒性.热带作物学报,30(6):730-734.
(Li G H, Yue H, Pang Y W, et al. 2009. Cold resistance of macadamia introduced to Xishuangbanna. Chinese Journal of Tropical Crops, 30(6):730-734. [in Chinese] )
李合生,孙群,赵世杰,等.2000.植物生理生化实验原理和技术.北京:高等教育出版社,260-261.
(Li H S, Sun Q, Zhao S J, et al. 2000. Principles and techniques of plant physiological biochemical experiment. Beijing: Higher Education Press, 260-261. [in Chinese] )
李守勇,孙明高,李学宏,等.2003.11个黑杨无性系物候期变异分析.西北林学院学报,18(3):40-42.
(Li S Y, Sun M G, Li X H, et al. 2003. Phenological phases of 11 poplar clones. Journal of Northwest Forestry College, 18(3):40-42. [in Chinese] )
李小琴,彭明俊,段安安,等.2012.低温胁迫对8个核桃无性系抗寒生理指标的影响.西北林学院学报,27(6):12-15.
(Li X Q, Peng M J, Duan A A, et al. 2012. Effects of low temperature stress on physiological indices of eight cold resistance juglans sigillata clones. Journal of Northwest Forestry University, 27(6):12-15. [in Chinese] )
柳新红,何小勇,苏冬梅,等.2007.翅荚木种源抗寒性综合评价体系的构建与应用.林业科学,43(10):45-50.
(Liu X H, He X Y, Su D M, et al. 2007. Establishment and application of integrated assessment system on cold resistance of different zenia insignis provenances. Scientia Silvae Sinicae, 43(10):45-50. [in Chinese] )
吕跃东,董凤祥,王贵禧,等.2008.平欧杂交榛抗寒性综合评价体系的建立与应用.林业科学,44(9):31-35.
(Lü Y D, Dong F X, Wang G X, et al. 2008. Establishment and application of integrated assessment system on cold resistance of hybrid hazelnut. Scientia Silvae Sinicae, 44(9):31-35. [in Chinese] )
庞磊,周小生,李叶云,等.2011.应用叶绿素荧光法鉴定茶树品种抗寒性的研究.茶叶科学,31(6):521-524.
(Pang L, Zhou X S, Li Y Y, et al. 2011. Study and identification of cold resistance of camellia sinensis by chlorophyll fluorescence method. Journal of Tea Science, 31(6):521-524. [in Chinese] )
苏晓华,丁昌俊,马常耕.2010.我国杨树育种的研究进展及对策.林业科学研究,23(1):31-37.
(Su X H, Ding C J, Ma C G. 2010. Research progress and strategies of poplar breeding in China. Forest Research, 23(1):31-37. [in Chinese] )
王晶英,敖红.2003.植物生理生化实验技术与原理.哈尔滨:东北林业大学出版社,11-13; 132-133.
谢吉荣,向邓云,谈峰.2002.南方红豆杉抗寒性生理指标的主分量分析.重庆师范学院学报: 自然科学版,19(2):50-52.
(Xie J R, Xiang D Y, Tan F. 2002. The principal component analysis of physiological targets of Taxus chinensis VAR. Mairei. Journal of Chongqing Normal University (Natural Science Edition), 19(2):50-52. [in Chinese] )
杨敏生,王春荣,裴保华.1997.白杨杂种无性系的抗寒性.东北林业大学学报,25(4):20-23.
(Yang M S, Wang C R, Pei B H. 1997. Cold resistance of hybrid clone of white poplar. Journal of Northeast Forestry University, 25(4):20-23. [in Chinese] )
杨向娜,魏安智,杨途熙,等.2006.仁用杏3个生理指标与抗寒性的关系研究.西北林学院学报,21(3):30-33.
(Yang X N, Wei A Z, Yang T X, et al. 2006. Studies on relationships between soluble protein contents,SOD and POD activity and cold resistant ability of 3 apricot varieties. Journal of Northwest Forestry University, 21(3):30-33. [in Chinese] )
杨志岩,彭建东,张妍,等.2010.杨树品种变温胁迫试验及抗冻性评价.林业科技开发,24(6):29-33.
(Yang Z Y, Peng J D, Zhang Y, et al. 2010. Influences of low temperature on freezing injury and assessment on cold resistance of poplar. China Forestry Science and Technology, 24(6):29-33. [in Chinese] )
张军,杨敏生,张钢,等.2011.新疆野苹果天然群体抗寒性的电阻抗图谱分析.林业科学,47(4):20-26.
(Zhang J, Yang M S, Zhang G, et al. 2011. Assessing frost hardiness of malus sieversii natural populations by electrical impedance spectroscopy. Scientia Silvae Sinicae, 47(4):20-26. [in Chinese] )
张倩,刘崇怀,郭大龙,等.2013.5个葡萄种群的低温半致死温度与其抗寒适应性的关系.西北农林科技大学学报: 自然科学版,41(5):149-154.
(Zhang Q, Liu C H, Guo D L, et al. 2013. Relationship between LT_(50) and cold adaptability of five grape varieties. Journal of Northwest A & F University(Natural Science Edition), 41(5):149-154. [in Chinese] )
中国科学院上海植物生理研究所,上海市植物生理学会.1999.现代植物生理学实验指南.北京:科学出版社,303.
Azzarello E, Mugnai S, Pandolfi C, et al. 2009. Comparing image (fractal analysis) and electrochemical (impedance spectroscopy and electrolyte leakage) techniques for the assessment of the freezing tolerance in olive. Trees, 23(1):159-167.
Deans J D, Billington H L, Harvey F J, et al. 1995. Assessment of frost damage to leafless stem tissues of Quercus petraea: a reappraisal of the method of relative conductivity. Forestry, 68:25-34.
Gleeson D, Walter M A L, Parkinson M. 2005.Overproduction of proline in transgenic hybrid larch (Larix×leptoeuropaea (Dengler)) cultures renders them tolerant to cold, salt and frost. Molecular Breeding, 15(1):21-29.
Kim S I, Tai T H. 2011. Evaluation of seedling cold tolerance in rice cultivars: a comparison of visual ratings and quantitative indicators of physiological changes. Euphytica, 178(3):437-447.
Lin K H, Hwang W C, Lo H F. 2007.Chilling stress and chilling tolerance of sweet potato as sensed by chlorophyll fluorescence. Photosynthetica, 45(4):628-632.
Li Yaqing, Zhang Gang, Que Shupeng, et al. 2009. Relationship between parameters of electrical impedance spectroscopy and frost hardiness in stems and needles of Pinus bungeana. Frontiers of Forestry in China, 4(2):242-248.
Qin Xuyin, Liu Yang, Mao Shanjing, et al.2011. Genetic transformation of lipid transfer protein encoding gene in Phalaenopsis amabilis to enhance cold resistance. Euphytica, 177(1):33-43.
Tanaka Y, Brotherton P, Hostetter S, et al.1997.The operational planting stock quality testing program at Weyerhaeuser.New Forests,13:423-437.
Uemura M,Warren G,Steponkus P L. 2003. Freezing sensitivity in the sfr 4 mutant of Arabidopsis is due to low sugar content and is manifested by loss of osmotic responsiveness. Plant Physiology, 131(4):1800-1807.
Wang Weijuan, Chen Yuzhen, Liu Meiqin, et al. 2008. Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings. Forestry Studies in China, 10(2):101-106.
Wanner L A, Junttila O.1999. Cold induced freezing tolerance in Arabidopsis. Plant Physiology, 120(2):391-400.
Xin Zhanguo, Li P H.1993. Relationship between proline and abscisic acid in the induction of chilling tolerance in Maize suspension cultured Cells. Plant Physiology, 103(2):607-613.
Zhang Dongfang, Zhang Zhiyi, Zhu Zhiti.2005. Cross breeding of Populus and its hybrids for cold resistance. Forestry Studies in China, 7 (3): 74-80.

[1]	Liu Jiming, Chen Zhong, Sun Caowen, Wang Lianchun, He Qiuyang, Dai Tengfei, Yao Na, Gao Shilun, Zhao Guochun, Shi Shuanglong, Jia Liming, Weng Xuehuang. Variation in Fruit and Seed Properties and Comprehensive Assessment of Germplasm Resources of the Genus Sapindus [J]. Scientia Silvae Sinicae, 2019, 55(6): 44-54.
[2]	Yang Baoshi, Guo Yingkai, Pang Kangying, Chen Yingjian, Zhu Yixin. Bearing Performance of Thin-Walled Timber Composite Cee-Sections [J]. Scientia Silvae Sinicae, 2019, 55(6): 111-121.
[3]	Li Jianqing, Mei Zengxia, Yang Zhongqi. Geostatistical Analysis on Spatial Distribution Pattern of Batocera horsfieldi (Coleoptera: Cerambycidae) Populations in Different Poplar Forest Types [J]. Scientia Silvae Sinicae, 2018, 54(3): 83-90.
[4]	Hou Junfeng, Bao Yongze, Zhou Yongdong. Effects of Superheated Steam Pretreatment on Conventional Drying of 50 mm-Thickness Poplar Lumber [J]. Scientia Silvae Sinicae, 2018, 54(2): 131-136.
[5]	Jiang Wenhu, Zhang Dejian, Liu Junxia, Li Chaoli, Lu Zhanyuan, Yang Minsheng. Comparative Analysis of Arthropod Communities in Transgenic Bt and Non-Transgenic Poplar-Cotton Composite Systems [J]. Scientia Silvae Sinicae, 2018, 54(10): 73-79.
[6]	Huang Juan, Chen Cun, Zhang Weixi, Ding Changjun, Su Xiaohua, Huang Qinjun. Effects of Drought Stress on Anatomical Structure and Photosynthetic Characteristics of Transgenic JERF36 Populus alba×P. berolinensis Seedling Leaves [J]. Scientia Silvae Sinicae, 2017, 53(5): 8-15.
[7]	Chu Wenyuan, Wang Yujiao, Zhu Dongyue, Chen Zhu, Yan Hanwei, Xiang Yan. Selection of Novel Reference Genes in Poplar under Salt and Drought Stresses [J]. Scientia Silvae Sinicae, 2017, 53(10): 70-79.
[8]	Xu Maosong, Lü Wenhua, Wenhua Wang. Properties of Strengthening-Dyeing-Combined Modified Plantation Poplar Wood [J]. Scientia Silvae Sinicae, 2017, 53(1): 82-87.
[9]	Cao Guoyu, Li Jixiang, Maimaitikuerban·Ali, Li Kaihua, Hu Jianjun. An Elite Variety of Populus deltoides ‘Beiyang’ [J]. Scientia Silvae Sinicae, 2016, 52(9): 156-156.
[10]	Huang Juan, Li Dan, Wang Ningning, Su Xiaohua, Huang Qinjun. Differences in Photosynthetic Characteristics and Growth in Short-Rotation Poplar of Different Spacing Configurations [J]. Scientia Silvae Sinicae, 2016, 52(8): 131-137.
[11]	Chen Panfei, Ren Yachao, Zhang Jun, Wang Jinmao, Yang Minsheng. Expression and Transportation of Bt Toxic Protein in 8-Year-Old Grafted Transgenic Poplar [J]. Scientia Silvae Sinicae, 2016, 52(7): 46-52.
[12]	Wang Wenbo, Wang Yanping, Wang Huatian, Ma Xuesong, Yi Wenhui. Effects of Different Continuous Cropping and Rotation of Poplar Plantation on Soil Nitrogen Bacteria Community and Nitrogen Metabolism [J]. Scientia Silvae Sinicae, 2016, 52(5): 45-54.
[13]	Lü Yuedong, Wang Guixi, Duan Wenbiao. Physiological differences in Dormancy of Different Cold Hardiness Hazelnut Hybrid [J]. Scientia Silvae Sinicae, 2016, 52(4): 142-146.
[14]	Yao Yanwu, Li Yongli, Liu Guobin, Cao Jun. An Elite Variety of Euonymus japonicus ‘Huayuanfa’ [J]. Scientia Silvae Sinicae, 2016, 52(4): 148-148.
[15]	Li Kaihua, Hu Jianjun. An Elite Variety of Populus deltoides ‘Chuangxin’ [J]. Scientia Silvae Sinicae, 2016, 52(3): 130-130.

Establishment of an Integrated Assessment System on Cold Resistance of Poplars at the Seedling Stage

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments