台湾杉遗传多样性的ISSR分析

doi:10.11707/j.1001-7488.20140608

Abstract

Abstract:

The genetic diversity and genetic structure of four natural populations of Taiwania cryptomerioides were investigated using inter-simple sequence repeat (ISSR). Of the 100 primers screened, 10 primers were selected and they generated 78 stable and distinct bands, among which 61 bands were polymorphic. The result of POPGENE indicated that T. cryptomerioides had a medium level of genetic variation at the species (the percentage of polymorphic bands PPB=78.21%, Nei's gene diversity H=0.249 4, Shannon's information index I=0.377 8) and population levels (PPB=52.56%, H=0.178 3, I=0.267 7). There was to a certain level genetic differentiation among four populations (G_ST: 0.285 1; Shannon's genetic differentiation: 29.14%; AMOVA genetic differentiation: 28.31%), and most of the genetic differentiation occurred within populations. The UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical and genetic distance(r=0.043 3, P=0.435 6). Based on these findings, we propose appropriate conservation strategies for this endangered species.

Key words: Taiwania cryptomerioides, ISSR, genetic diversity, genetic structure, conservation strategy

CLC Number:

Li Jiangwei, Yang Qinjun, Liu Xiuqun, Ji Hua, Zhang Zhuowen. Genetic Diversity of Taiwania cryptomerioides Detected by ISSR Analysis[J]. Scientia Silvae Sinicae, 2014, 50(6): 61-66.

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Genetic Diversity of Taiwania cryptomerioides Detected by ISSR Analysis

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