哈茨木霉T28发酵液提取物对致病疫霉的抑菌作用及有效成分

doi:10.11707/j.1001-7488.20130717

Abstract

Abstract:

Six extracts were obtained from the fermentation liquid of Trichoderma harzianum strain T28 by using different extract methods and organic solvents with different polarity. Inhibiting activities of the extracts on Phytophthora infestans were tested. The extracts with high inhibiting activities were further analyzed with GC-MS. The results indicated that the extracts obtained with step-by-step extraction method and ethyl acetate extract had the optimum inhibiting effect on mycelial growth, sporangium germination and sporangium released zoospores of P. infestans, with the inhibition rates of 67.06 percent, 71.71 percent and 76.25 percent, respectively. Ethyl acetate-extracted ingredients in the sequential extraction and the direct extraction process were analyzed with GC-MS, and 10 and 15 components were identified, respectively; the same components in the two extraction methods included 2,5-di-tert-butylphenol, di-tert-butyl phthalate, diisobutyl phthalate, eighteen methyl carbonate, dibutyl phthalatel and mono-2-ethylhexyl Phthalate. These components might be the active chemical ingredients which inhibited P. infestans.

CLC Number:

S763.15

Yang Libin, Song Ruiqing, Deng Xun, Li Chongwei. Active Components of Extracts from the Fermentation Liquid of Trichoderma harzianum Strain T28 and Their Inhibiting Activities to Phytophthora infestans[J]. Scientia Silvae Sinicae, 2013, 49(7): 118-122.

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Active Components of Extracts from the Fermentation Liquid of Trichoderma harzianum Strain T28 and Their Inhibiting Activities to Phytophthora infestans

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