外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响

doi:10.11707/j.1001-7488.20130609

Abstract

Abstract:

In this paper, mature zygotic embryo of Sorbus pohuashanensis were used to study effects of an exogenous nitric oxide donor (sodium nitroprusside, SNP), NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), abscisic acid (ABA) and 2,5-norbornadiene (NBD)—an inhibitor of the ethylene receptor on the embryo germination and reactive oxygen species (ROS) accumulation at early stage of the seedling development by the petri dish germination method indoors. The results showed that the inhibitory effect of PTIO or ABA on the embryo germination was relieved by the SNP pretreatment under light. However, the SNP pretreatment did not influence the inhibitory action of NBD to embryo germination. The SNP pretreatment increased the chlorophyll content in leaves of seedlings, which was not removed by PTIO treatment. The contents of hydrogen peroxide (H₂O₂) and superoxide anion in seedling leaves were increased by SNP pretreatment, which was cancelled by PTIO treatment. The PTIO treatment reduced the superoxide anion content in seedling roots, which was removed by SNP pretreatment. The results would lay the foundation for interpreting the relationships between reactive nitrogen species (RNS) mediated (NO-dependent) dormancy removal and ROS (H₂O₂ and superoxide anion) accumulation in the embryo germination.

Key words: Sorbus pohuashanensis, embryo germination, nitric oxide, abscisic acid, ethylene

CLC Number:

S718.43

Yang Ling, Wang Jianan, Bian Lei, Shen Hailong. Effects of Exogenous Nitric Oxide on Embryo Germination and ROS Accumulation in Seedling Growth Initial Stage of Sorbus pohuashanensis[J]. Scientia Silvae Sinicae, 2013, 49(6): 60-67.

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Effects of Exogenous Nitric Oxide on Embryo Germination and ROS Accumulation in Seedling Growth Initial Stage of Sorbus pohuashanensis

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