沙冬青细胞对MeJA处理的初始生理响应

doi:10.11707/j.1001-7488.20121005

Scientia Silvae Sinicae ›› 2012, Vol. 48 ›› Issue (10): 24-29.doi: 10.11707/j.1001-7488.20121005

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Primary Physiological Responses of Ammopiptanthus mongolicus Cells Induced by Methyl Jasmonate

Gao Haibo^1,2

1. College of Life Science, Linyi University Linyi 276005;2. College of Biological Science and Biotechnology, Beijing Forestry University Beijing 100083

Received:2012-04-25 Revised:2012-06-06 Online:2012-10-25 Published:2012-10-25

Abstract

Abstract:

This study investigated changes in Ca²⁺ flux, hydrogen peroxide content and membrane potential of Ammopiptanthus mongolicus cells in response to methyl jasmonate treatment. Results showed that methyl jasmonate caused proton influx, hydrogen peroxide production and membrane depolarization, which were completely inhibited by pretreatment with intracellular calcium store release inhibitor ruthenium red, demonstrating that calcium release from intracellular calcium store was necessary for proton influx, hydrogen peroxide production and membrane depolarization. Both proton influx and membrane depolarization, triggered by treatment with methyl jasmonate, were inhibited by pretreatment with a hydrogen peroxide scavenger, indicating that hydrogen peroxide production was also necessary for proton influx which was the main reason of membrane depolarization.

Key words: methyl jasmonate, Ammopiptanthus mongolicus, Ca²⁺ ion flux, hydrogen peroxide, membrane potential

CLC Number:

Gao Haibo;. Primary Physiological Responses of Ammopiptanthus mongolicus Cells Induced by Methyl Jasmonate[J]. Scientia Silvae Sinicae, 2012, 48(10): 24-29.

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Primary Physiological Responses of Ammopiptanthus mongolicus Cells Induced by Methyl Jasmonate

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