Abstract:

In this paper, I investigated impacts of four storage temperatures (room temperature,4 ℃,-10 ℃ and -20 ℃) and six storage times (0,72,144,216,286 and 358 days) on Pinus massoniana pollen germination and activities of SOD,POD and CAT. Results showed that P. massoniana pollen could remain a high germination proportion and strong resistance to oxidation for 72 days at the room temperature,216 days at 4 ℃ and 286 days at -10 ℃ or -20 ℃ respectively. During storage period,the germination proportion of pollen decreased significantly when the activities of SOD,POD and CAT were around the highest levels. The three enzymes had different responses to the storage temperature. CAT was a responding protection enzymes, but SOD and POD were non responding ones at room temperature or 4 ℃; however, POD was a responding protection enzymes, whereas SOD and CAT were non responding ones at -10 or -20 ℃.

Key words: Pinus massoniana, pollen, SOD, POD, CAT