Abstract:

In order to study the relationship between secondary metabolism substances in poplar and resistance of Saperda populnea L. to the substances, the insects were fed with 10 kinds of secondary metabolism substances, selected based on previous researches. The larvae mortality and activities of enzymes, including SOD, CAT, glutathione s-transferase, carboxylesterase and α-napthyl acetate, in the larvae were investigated. Results showed that 2.00 mg ·g^-1 and 20.00 mg ·g^-1 syringic acid, 16.00 mg ·g^-1 phenol, 8.00 mg ·g^-1 trans-cinnamic acid, 50.00 mg ·g^-1 4-hydroxybenzoic acid and 4.00 mg ·g^-1 chlorogenic acid increased mortality of the larvae. Meanwhile the activity of SOD in larvae increased significantly, after being fed with secondary metabolism materials. The activity of SOD of larvae increased significantly after fed with secondary metabolism materials, and the activity of α-napthyl acetate was increased by tannin. However, secondary metabolism materials had no obvious effect on CAT and glutathione S-tramsferases of S. populnea.

Key words: Saperda populnea, secondary metabolism materials, poplar, artificial feeding, enzymatic activity