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Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (5): 171-176.doi: 10.11707/j.1001-7488.20110529

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Transformation of TCS Gene to Paulownia elongata and Its Virus Resistance

Liu Jing1, Huang Yanyan1, Weng Manli2, Luo Lei1, Zhang Hong1, Wang Changxian1, Niu Qinglin3, Feng Dianqi1   

  1. 1. Taishan Institute of Forestry Science Tai'an 271000;2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101;3. Shandong Agricultural University Tai'an 271018
  • Received:2010-06-03 Revised:2010-11-30 Online:2011-05-25 Published:2011-05-25

Abstract:

In this study, the trichosanthin (TCS) gene, which was cloned from Trichosanthes kirilowii, was transformed into Paulownia elongata through agrobacterium-mediated method. The conversion rate was 29.2% with 86 transformed strains obtained. The target gene had approximately 50% positive expression rate in plantlets through 11 times subculture and PCR detection for 4 times. And the PCR detection of the plants in field showed that the positive rate was between 40% and 50%. No positive expression of the labeled product of witches' broom was found in the transformed plants after infection susceptible experiment and molecular detection, meanwhile, the virus infection rate of the control was up to 15.0%. Thus, it indicated that, compared to the control, the transformed plants showed higher resistance to the witches' broom disease.

Key words: Paulownia elongata, TCS gene, agrobacterium-mediated, stability, virus resistance

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