欧洲榛微卫星对我国榛属种质资源的分析

doi:10.11707/j.1001-7488.20090204

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (2): 22-26.doi: 10.11707/j.1001-7488.20090204

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Genetic Diversity of Six Corylus Species in China Detected with Microsatellite Isolated from Corylus avellana

Cheng Lili¹,Huang Wugang¹,Zhou Zhijun²,Liu Jianli³,Wang Yanmei³,Su Shuchai³,Zhai Mingpu³

（1. Beijing Institute of Forestry and Pomology,Beijing Academy of Agricultural and Forestry Sciences Beijing 100093;2. Changping District Forest Department Beijing 102200; 3. Beijing Forestry University Beijing 100083）

Received:2008-02-01 Revised:1900-01-01 Online:2009-02-25 Published:2009-02-25

Abstract

Abstract:

Abstract: Diversity of 6-Corylus-species distributed in China was analyzed by 11 SSR markers isolated from C. avellana. A total of 81 alleles were amplified by 11 high informative SSR primer pairs from 34 DNA templates of the accessions tested. Number of alleles per locus ranged from 4 to 13 with an average value of 7.36. Average value of polymorphic information content (PIC) was 0.73,ranged from 0.47 to 0.86 Sequencing the PCR amplified DNA showed there existed microsatellites of C.avellana in 6-Corylus-species. Other short tri-nucleotide repeat sequence also exhibited variable in repeat numbers at CAC C28 locus. In addition,the average Nei's gene diversity index was 0.59 for all the loci of three species, including Corylus heterophylla,Corylus mandshurica and Corylus heterophylla var. sutchuenensis, which were of potential commercial value. This study demonstrated that the silver-staining SSR was a useful tool for genetic diversification, source conservation, variety identification and construction of molecular map among species of the genus Corylus.

Key words: Key words: Corylus, SSR marker, polymorphism, DNA sequencing

Cheng Lili;Huang Wugang;Zhou Zhijun;Liu Jianli;Wang Yanmei;Su Shuchai;Zhai Mingpu. Genetic Diversity of Six Corylus Species in China Detected with Microsatellite Isolated from Corylus avellana[J]. Scientia Silvae Sinicae, 2009, 12(2): 22-26.

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Genetic Diversity of Six Corylus Species in China Detected with Microsatellite Isolated from Corylus avellana

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