南华松叶蜂病毒DnNPV的发现及其毒力测试

doi:10.11707/j.1001-7488.20070724

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (07): 138-141.doi: 10.11707/j.1001-7488.20070724

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Finding of Nuclear Polyhedrosis Virus of Diprion nanhuaensis and Its Virulence Test

Yang Miaomiao^1,2,Li Menglou¹,Qu Liangjian²,Wang Yuzhu²,Su Qin³,Zhang Yong'an²

1.College of Forestry,Northwest A&F University Yangling 712100; 2. Institute of Forest Ecology, Environment and Protection, CAF Key Lab. Of Forest Protection of State Forestry Administration Beijing 100091; 3. People's Government of Zhangcheng Town, Longde, Ningxia Longde 635400

Received:2006-07-25 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

Abstract

Abstract:

One virus of Diprion nanhuaensis was separated from the died D. nanhuaensis, the virus belongs to nuclear polyhedrosis virus, bacilliform virus particle in inclusion body appears SNPV(single nucleocapsid polyhedrosis virus) by electron microscopy. The structure of inclusion body appears polyhedron and close to rotundity, and the length of diameter of inclusion body ranges 0.5～1.0 μm, a little proportion of them shows anomalistic form. There are many bacilliform virus particles in one inclusion body. Six virus particles in one inclusion body take up a large proportion and one virus particle in one inclusion body only takes up a small proportion, the length of virus particle ranges 125～200 nm,and the length of diameter of virus particle ranges 25～30 nm. Meanwhile,the virus can infect the 2～3 instars Neodiprion xiangyunicus and the 2～3 instars D. nanhuaensis in laboratory.

Key words: Diprion nanhuaensis, nuclear poyhedrosis virus, ultrastructure, Neodiprion xiangyunicus, Diprion nanhuaensis

Yang Miaomiao;Li Menglou;Qu Liangjian;Wang Yuzhu;Su Qin;Zhang Yong'an. Finding of Nuclear Polyhedrosis Virus of Diprion nanhuaensis and Its Virulence Test[J]. Scientia Silvae Sinicae, 2007, 43(07): 138-141.

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Finding of Nuclear Polyhedrosis Virus of Diprion nanhuaensis and Its Virulence Test

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