Abstract:

Several kinds of important enzyme systems in Tetranychus viennensis treated with chloroform extract of Kochia scoparia were tested. The results indicated that the extracts of K. scoparia had strongly acaricidal activities against T. viennensis. The activities of glutathione-S-transferase and carboxylesterase in T. viennensis, which are all very important enzyme systems and participate in metabolizing and detoxification for inter-origin and foreign-origin poison especially pesticide in mite, were strongly induced. This result indicated that there are some effective components against T. viennensis in extract of K.scoparia which could arouse detoxification of enzyme system. But the activities of monoamine oxidase and Ca²⁺ -ATPs were restrained. Been restrained of Ca²⁺ -ATPs could destroy the balance of Ca²⁺ which exist both in and out of the cell and influence the transmit and excitement of neurone. Been restrained of monoamine oxidase could result in accumulating of neural amic and cause the transmit block of nerve in mites. The result from the experiment also indicated that the discovery open up a good developing prospect for making full use of K. scoparia as new resources of botanical acaricide.

Key words: Kochia scoparia, Tetranychus viennensis, activity of enzyme