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Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (1): 29-36.doi: 10.11707/j.1001-7488.20060106

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Isolation and Structural Identification of the Anthocyanins from the Flower Color Pigment of Prunus mume ‘Nanjing Hongxu’ (Nanjing Red-Bearded)

Zhao Changling1,2,Guo Weiming3,Chen Junyu4   

  1. 1.College of Life Sciences, Nanjing Agricultural University Nanjing210095; 2.College of Agricultural Sciences and Biotechnology,Yunnan Agricultural University Kunming650201; 3.College of Horticulture, Nanjing Agricultural University Nanjing210095;4.College of Landscape Architecture, Beijing Forestry University Beijing100083
  • Received:2004-06-15 Revised:1900-01-01 Online:2006-01-25 Published:2006-01-25

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Abstract:

Two major anthocyanins of the flower color pigment of Prunus mume‘Nanjing Hongxu’ were isolated with MeOH-HOAc-H2O (10∶1∶9, V/V), and purified by paper chromatography and subsequent column chromatography. Specific chemical reactions, TLC, PC, UV-Vis spectra, HPLC, GC, 1H NMR,13C NMR and FAB MS analyses indicate that the anthocyanins are cyanidin 3-O-(6″-O-α-rhamnopyranosyl-β-glucopyranoside) and cyanidin 3-O-(6″-O-galloyl-3″-O-β-glucopyranosyl-β-glucopyranoside) respectively. In addition to contributing to the mauve flower color, the anthocyanins may improve the ability of P. mume ‘Nanjing Hongxu’ to survive in cold.

Key words: Prunus mume “Nanjing Hongxu&rdquo, (Nanjing red-bearded);flower color pigment;anthocyanin;molecular structure