Abstract:

The study was undertaken to evaluate the anti-fertility effect of the extract chloroform isolated from neem oil. Adult male Kunming mice received 2 g\5kg^-1 the extract chloroform orally for one week. The data from fertility tests showed that the extracts chloroform from neem oil has positive antifertility effect,the LD₅₀ is 33 g·kg^-1, the level of aminotransferase increase in first to third months after treated with extracts chloroform, but turn back gradually in 4-6th months, serum testosterone level has no obviously alteration. The flow cytometry data revealed that extract chloroform could cause a decrease in seminiferous cells number,and an increase in the apoptosis rate. The concentration of total alkaline nuclear protein (TNBP ) was decreased by 47.11% and the ratio of total tissue protein (TH)/ spermic nuclear protein (RP) was increased by 80.25%. The action of anti_fertility may be relevant with interferring the mediated immunity in the metamorphous stage. It is showed that a new way method of controling mouse calamity can be developed.

Key words: neem oil, extract chloroform, anti-ferlitily, calamity of mice