Abstract:

After extracting Bursaphelenchus xylophilus and B. mucronatus genomic DNA by SDS method, ten strains of B. xylophilus and ten strains of B. mucronatus from some epidemic areas were compared in the relationships by selected six primers through RAPD assay. The result of RAPD could discern obviously B. xylophilus from B. mucronatus and the result is consistent with morphology analysis. The cluster analysis also demonstrated between interspecific and intraspecific variations, which interspecific variations were more than intraspecific variations. Nematode homology in the same and adjacent district is higher than in distant district. In the same district host has very little effect on the nematode distribution. The method is sensitive and accurate, and can be regarded as a reliable technique for the study of nematode distribution, spread pathway, detection and prevention.

Key words: Bursaphelenchus xylophilus, B. mucronatus, RAPD, cluster analysis