银杏胚轴、子叶诱导胚状体发生及成苗的研究

doi:10.11707/j.1001-7488.20050231

Scientia Silvae Sinicae ›› 2005, Vol. 41 ›› Issue (2): 178-181.doi: 10.11707/j.1001-7488.20050231

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Study on Somatic Embryogenesis of Embryonic Axis and Cotyledon in Ginkgo biloba and Plantlet Formation

Guo Changlu^1,2,Chen Ligeng¹,He Xinhua^1,3,Syed Asgar Tori¹,Yuan Haiying¹

1.Key Laboratory of Ministry of Agriculture for Growth Development and Biotechnology of Horticultural Plant, Zhejiang University Hangzhou 310029; 2.School of the Ocean,Harbin institute of technology Weihai 264209;3.Agricultural College of Guangxi University Nanning 530005

Received:2003-10-31 Revised:1900-01-01 Online:2005-03-25 Published:2005-03-25

Abstract

Abstract:

Embryonic axis and cotyledon of immature and mature embryos were induced as explants for embryogenesis in Ginkgo biloba. Results showed somatic embryos could be induced only through embryonic axis and cotyledon of immature embryos. MK + NAA 1 mg·L^-1 + 6-BA 1 mg·L^-1 was the best medium for somatic embryogenesis from embryonic axis, and somatic embryogenesis rate was 45.28%. MK + NAA 1.5 mg·L^-1 + 6-BA 1 mg·L^-1 was the best medium for somatic embryogenesis from cotyledon, and somatic embryogenesis rate was 12.9%. The growth and development of somatic embryos were promoted by adding 10% coconut milk into MK, and 34.48% somatic embryos could develop into plants.

Key words: Ginkgo biloba, embryonic axis, cotyledon, somatic embryogenesis, plant generation

Guo Changlu;Chen Ligeng;He Xinhua;Syed Asgar Tori;Yuan Haiying. Study on Somatic Embryogenesis of Embryonic Axis and Cotyledon in Ginkgo biloba and Plantlet Formation[J]. Scientia Silvae Sinicae, 2005, 41(2): 178-181.

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Study on Somatic Embryogenesis of Embryonic Axis and Cotyledon in Ginkgo biloba and Plantlet Formation

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