Scientia Silvae Sinicae ›› 2005, Vol. 41 ›› Issue (2): 48-52.doi: 10.11707/j.1001-7488.20050208
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Lü Shoufang,Zhang Shougong,Qi Liwang,Sun Xiaomei,Wang Jianhua
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Embryogenic cultures were initiated from immature zygotic embryos of Larix kaempferi on S culture medium containing 6-BA 0.5 mg·L-1 , KT 0.5 mg·L-1 and 2,4-D 1.0 mg·L-1 .Embryogenic callus were subcultured and multiplicated on S culture medium containing dropping off each hormone concentration, 6-BA 0.25 mg·L -1 , KT 0.25 mg·L-1 and 2,4-D 0.1 mg·L-1 .We set up 3 steady-going embryogenic cell lines, such as ‘riyong10',‘riyong 69',‘ricao 34'.Somatic embryos obtained on S culture medium with ABA 15 mg·L -1 and PEG4000 100 g ·L-1 . These three culture media were added glutamine 450 mg·L-1 , casein hydrolysate 500 mg·L -1 , sucrose 30 g·L-1 and gellan gum 3 g·L-1 (Sigma ),pH5.8.Mature somatic embryos can germinate and develop further into plantlets when they are isolated and cultured on a hormone-free S culture medium adding sucrose 25 g·L-1 and gellan gum 3 g·L-1 (Sigma# ),pH5.8.
Key words: Larix kaempferi, somatic embryogenesis, culture medium, embryogenic callus, mature somatic embryos
Lü Shoufang;Zhang Shougong;Qi Liwang;Sun Xiaomei;Wang Jianhua. Somatic Embryogenesis from Immature Embryos of Larix kaempferi[J]. Scientia Silvae Sinicae, 2005, 41(2): 48-52.
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URL: http://www.linyekexue.net/EN/10.11707/j.1001-7488.20050208
http://www.linyekexue.net/EN/Y2005/V41/I2/48