NAA和IBA对四倍体刺槐试管苗生根影响及不定根发育过程解剖观察

doi:10.11707/j.1001-7488.20040313

Scientia Silvae Sinicae ›› 2004, Vol. 40 ›› Issue (3): 75-79.doi: 10.11707/j.1001-7488.20040313

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Effect of NAA and IBA on Rooting of Tetraploid Robinia pseudoacacia in vitro and Anatomical Observation on Adventitious Root Development

Li Yun,Wang Shuzhi,Tian Yanting,Qian Yongqiang

College of Biological Sciences and Technology,BFU Beijing100083；Institute of Archaeology, Academy of Social Science Beijing100710

Received:2002-09-16 Revised:1900-01-01 Online:2004-05-25 Published:2004-05-25

Abstract

Abstract:

The effect of NAA, IBA on rooting of shoots in test tubes of the tetraploid Robinia pseudoacacia was studied by the aid of the different test designs such as factorial experiment, regressive orthogonality test. The optimal medium was 1/2 MSIBA 0.4 mg·L^-1NAA 0.25 mg·L^-1sucrose 2%agar 0.6%. The adventitious root formation of shoots in test tube was also observed by means of the anatomical method. Latent root primordium was not found in shoots in test tubes. The induced root primordium which originated from the division and differentiation of pith ray cell developed into the adventitious roots.

Key words: Robinia pseudoacacia, Tetraploid, Tissue culture, Adventitious roots, Anatomical structure

Li Yun;Wang Shuzhi;Tian Yanting;Qian Yongqiang. Effect of NAA and IBA on Rooting of Tetraploid Robinia pseudoacacia in vitro and Anatomical Observation on Adventitious Root Development[J]. Scientia Silvae Sinicae, 2004, 40(3): 75-79.

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Effect of NAA and IBA on Rooting of Tetraploid Robinia pseudoacacia in vitro and Anatomical Observation on Adventitious Root Development

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