Abstract:

In order to improve quality of somatic embryos and rooting frequency in Larix principis-rupprechtii,2,4-dichlorophenoxy acetic acid(2,4-D) was replaced by naphthalene acetic acid(NAA),which improved the quality of early stage embryos, and thus improved the formation of high quality somatic embryos. Subcultures on this maturation medium, containing 3% maltose to replace sucrose, together with 4% PEG4000 and 0.1% activated charcoal (AC), the maturation frequency of somatic embryos was not lower (106.7 embryos per gram embryogenic calli, EC), but the quality of somatic embryos was improved distinctly, such as the hypocotyl was longer and the rooting frequency was up to 57.89%. The root system formed grew normal. Woody plant medium (WPM) as germination medium for somatic embryos, containing phloroglucinol (PG) 6 mg·L^-1 was the best concerning embryo germination and rooting.

Key words: Larix principis-rupprechtii, Somatic embryos, Maltose, Hypocotyl