Abstract:

outstanding clones of Zanhuangdazao and Zanxindazao were studied using RAPD (random amplified polymorphic DNA) technique. This study aimed at finding out the molecular fingerprints of some related cultivars and clones. 16 10 mer primers selected from 80 ones were used for RAPD analysis. Of the total 70 bands amplified, 19 (27.14%) were polymorphic. RAPD fingerprints of 10 outstanding clones of Zanhuangdazao and Zanxindazao were established using 5 primers and 6 bands (OPE11～1 300 bp、OPG6～3 000 bp、OPF16～840 bp、OPA5～300 bp、OPA5～370 bp、OPE2～1030 bp). The dendrograms of cluster analy sis for Zanhuangdazao clones and Zanxindazao were also established by AVERAGE and SINGLE methods. At the same time, the RAPD analysis on related clones and varieties evolution was discussed accordingly.

Key words: Zanhuangdazao, Zanxindazao, Clones, RAPD