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Scientia Silvae Sinicae ›› 2001, Vol. 37 ›› Issue (zk): 137-142.doi: 10.11707/j.1001-7488.2001S124

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STUDY ON METABOLIZATION CHARACTERS OF CELL MEMBRANE OF CRYOPRESERVATION MATERIALS OF CASTANOPSIS CARLESII AND C. KAWAKAMII

Chen Liguang,Zheng Yushan,Qiu Erfa,Zhou Xiaohua,Wu Zhuoxi   

  1. Forestry College of Fujian Agriculture and Forestry University Nanping 353001
  • Received:2001-01-08 Revised:1900-01-01 Online:2001-11-25 Published:2001-11-25

Abstract:

By using different moisture content (MC), freezing and thawing methods, conservation materials of two tree spices, Castanopsis carlesii and C. kawakamii, were immersed in liquid nitrogen and then their germination, conductivity, SOD activity and MDA content were traced to measured. The results showed that in cryopreservation process, MC control was the main and firstly factor, and secondly were proper freezing methods and thawing methods. The optimal MC of both C. carlesii seeds and excised embryos were 12%MC, and MF-QT methods. Optimal MC of C. kawakamii seeds was15%MC, but of its excised embryos was properly down to 12%MC, MF-QT methods.

Key words: Cryopreservation, C. carlesii, C. kawakamii, Recalcitrant seeds