Abstract:

By means of cryopreservation(-196℃)pretreated with cryoprotectants or not, characteristics of seed quality and enzyme activity of embryos were measured and analyzed both before and after cryopreservation, and long term storage feasibility was studied. The results showed that moisture content (MC) was the main factors deciding the cryopreservation effects of both C. henryi seeds and excised embryos. Moreover, the treatment desiccating down to a medium MC should be taken in cryopreservation process. Under the conditions of no cryoprotectants pretreatment, the optimal group of treatments was 15%～20% MC,mild freezing, and quick thawing. Otherwise, enzyme activity protected by cryoprotectants was higher than that of none, and the optimal group of treatments was 20%～25%MC, mild freezing, and slow thawing.

Key words: Castanea henryi, Excised embryos, Cryopreservation, Enzyme activity, Cryoprotectants