Abstract:

Poplars was seriously attacked by insects usually in many areas and insect resistant poplars are expected in forest production. In the experiment, the modified Bacillus thuringiensis (Bt) δ-endotoxin gene and proteinase inhibitor(PI)gene were contransformed to the explants of Populus nigra mediated by Agrobacterium tumefaciens. The integration of Bacillus thuringiensis gene and proteinase inhibitor(PI)gene were confirmed by PCR analysis and Southern hybridization. The results of bioassay showed that the transgenic plants had toxicity to the larvae of Lymantria dispar L., and that the transgenic plants containing Bt gene and PI gene had enhanced toxicity to larvae by comparison with plants containing only Bt gene.

Key words: Populus nigra, Bt gene, Proteinase inhibitor gene, Insect-resistance