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林业科学 ›› 2006, Vol. 42 ›› Issue (1): 85-89.doi: 10.11707/j.1001-7488.20060115

• 论文及研究报告 • 上一篇    下一篇

球孢白僵菌对竹梢凸唇斑蚜的毒力

徐华潮1 施祖华2 吴鸿1   

  1. 1.浙江林学院森林保护研究所,临安311300;3.浙江大学植物保护系,杭州310029
  • 收稿日期:2003-11-11 修回日期:1900-01-01 出版日期:2006-01-25 发布日期:2006-01-25

Virulence of Beauveria bassiana against Takecallis taiwanus(Takahashi)

Xu Huachao1,Shi Zuhua2,Wu Hong1   

  1. 1.Institute of Forest Protection, Zhejiang Forestry College Lin'an 311300;2.Department of Plant Protection, Zhejiang University Hangzhou 310029
  • Received:2003-11-11 Revised:1900-01-01 Online:2006-01-25 Published:2006-01-25

摘要:

测试来自不同虫源的5个球孢白僵菌菌株对竹梢凸唇斑蚜的毒力。结果表明:5个菌株对竹梢凸唇斑蚜都表现出一定的致病力。根据时间-剂量-死亡率模型,考查不同菌株的剂量效应和时间效应的指标值lg(LC50)和LT50,菌株B12,Bxs,REBb01,Zhe-B和F-263在接种后第9天的lg(LC50)值分别为4.0549,4.4620,4.6261,4.9290和5.1580;在1×106个孢子·mL-1浓度下LT50分别为3.63,3.70,4.31,4.34和5.20d。根据该蚜虫累积死亡率随时间和剂量变化的三维曲面图综合评价,5个菌株的毒力从强到弱依次为B12,Bxs,REBb01,Zhe-B和F-263。

关键词: 竹梢凸唇斑蚜, 球孢白僵菌, 时间-剂量-死亡率模型, 毒力, 生物测定

Abstract:

Virulence bioassays of the 5 isolates of Beauveria bassiana from different species of insects against Takecallis taiwanus (Takahashi) were carried out. The result showed that all 5 isolates tested were capable of infecting T. taiwanus(Takahashi). The time-dose-mortality model (namely complementary log-log model) was used for the analysis of the data obtained from the bioassay. Time-dependent lg(LT50) and dose-dependent LC50 were estimated respectively. For B12, Bxs, REBb01, Zhe-B and F-263, the lg(LC50) by the 9th day after inoculation were estimated to be 4.054 9, 4.462 0, 4.626 1, 4.929 0 and 5.158 0 conidia·mL-1 respectively, and the LT50 at an inoculate dose of 1×106 conidia·mL-1 were estimated to be 3.63, 3.70, 4.31, 4.34 and 5.20 days respectively. Based on three-dimension figures of cumulative mortality of T. taiwanus(Takahashi) with the changes of time and dose, virulence of each isolate under different time and dose were evaluated integrally. The virulence of 5 isolates in a descending order was B12, Bxs, REBb01, and followed by Zhe-B and F-263.

Key words: Takecallis taiwanus (Takahashi), Beauveria bassiana, time-dose-mortality model, virulence, bioassay