关键词: RAPD, 遗传变异, 杉木, 种源, 种源检测

Abstract:

According to the work of national cooperative group of Chinese fir provenance test, we chose seven representative provenances from seven Chinese fir provenance zones. Seven provenance samples were randomly collected from Xinyang gene pool(Henan Province), total DNA was extracted and purified from leaves.In this paper, the optimum PCR reaction conditions for RAPD analysis were constructed and intensive RAPD analysis was conducted for above samples through more than 300 PCR reactions. The polymorphic DNA sequence analysis was conducted for seven provenances using twenty-three primers. The result showed that the proportion of polymorphic RAPD loci for seven provenances was 79.8%, the genetic distance for seven provenances ranged between 0.225 and 0.4407, averaging 0.322. Those results reveal high variation among seven provenances.The natural distribution of Chinese fir is important for provenance test and breeding, but it is difficult to deduce the natural distribution of Chinese fir, since the Chinese fir has covered complicated circumstance and has a long cultivation history. The dendrogram of seven provenances was constructed on the basis of genetic similarity, the tree showed the relationships among different provenances, Napo、Rongshui、Huitong and Jinping got together, Jianwo、Hongya、Shangcheng gathered respectively. The dendrogram shows the provenances from the western part of Nanlins mountains are more similar than other provenances, so we deduce the center-western part of Nanlings mountain is the central distribution zones of Chinese fir, this result is as same as the provenance test.

Key words: RAPD, Genetic variation, Chinese fir provenance, Provenance test