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林业科学 ›› 1999, Vol. 35 ›› Issue (3): 34-40.

• 论文及研究报告 • 上一篇    下一篇

树木溃疡病病原真菌类群分子遗传多样性研究Ⅰ.——小穴壳菌属、疡壳孢属、壳囊孢属、盾壳霉属分类地位的分子证明*

张星耀,赵仕光,朴春根,吕全,贾秀贞   

  1. 中国林业科学研究院森林生态环境与保护研究所 北京 100091
  • 收稿日期:1998-12-20 出版日期:1999-05-25 发布日期:1999-05-25
  • 基金资助:
    国家自然科学基金(39770617);国家林业局森林保护学重点开放实验室基金

MOLECULAR GENETIC DIVERSITY OF PATHOGENIC FUNGAL GROUP CAUSING TREE CANKER Ⅰ.——EVIDENCE OF TAXA FOR DOTHIORELLA, DOTHICHIZA, CYTOSPORA AND CONIOTHYRIUM IN MOLECULAR LEVEL

Xingyao Zhang,Shiguang Zhao,Chungen Piao,Quan Lü,Xiuzhen Jia   

  1. The Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091
  • Received:1998-12-20 Online:1999-05-25 Published:1999-05-25

摘要:

小穴壳菌属(Dothiorela Sac.)(有性阶段是葡萄座腔菌属Botryosphaeria Cas.)、疡壳孢属(Dothichiza Lib.)、壳囊孢属(Cytospora Ehrenb.)、盾壳霉属(Coniothyrium Sacc.)等4属真菌是引起树木溃疡病的主要病原真菌类群,是我国现阶段森林的重大有害生物。本文报道了这4个属的真菌基于28SrDNA-PCR-RFLP和RAPD解析的分类学研究结果, 15个供试菌株聚为4类,每一类即一属,在分子水平上证明了其属的传统分类地位的客观性。

关键词: 树木, 溃疡病病原真菌, 28S rDNA-PCR-RFLP, RAPD, 分类学

Abstract:

The fungal group, including the genera of Dothiorella Sacc. (its sexual phase is Botryosphaeria Cas.), Dothichiza Lib., Cytospora Ehrenb. and Coniothyrium Sacc. presently are the most important forest pathogens in China. In this paper, the tested fifteen isolates of the group are divided into four clusters by the methods of 28S rDNA-PCR-RFLP and RAPD analyses. Each cluster just corresponds to one genus. So, the objectivity of the traditional taxonomy of these genera is proved in the molecular level.

Key words: Tree, Canker pathogenic fungi, 28SrDNA-PCR-RFLP, RAPD, Taxonomy