欢迎访问林业科学,今天是

林业科学 ›› 2010, Vol. 46 ›› Issue (12): 144-152.doi: 10.11707/j.1001-7488.20101224

• 综合评述 • 上一篇    下一篇

植物细胞壁伸展蛋白研究述评

郭联华1,2, 尹佟明1, 李淑娴1, 黄敏仁1, 王明庥1   

  1. 1. 南京林业大学 江苏省杨树种质创新与品种改良重点实验室 南京 210037;2. 浙江农林大学林业与生物技术学院 临安 311300
  • 收稿日期:2010-06-30 修回日期:2010-09-28 出版日期:2010-12-25 发布日期:2010-12-25
  • 通讯作者: 尹佟明

A Review of Extensins in Plant Cell Wall

Guo Lianhua1,2, Yin Tongming1, Li Shuxian1, Huang Minren1, Wang Mingxiu1   

  1. 1. Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement Nanjing Forestry University Nanjing 210037;2. School of Forestry and Biotechnology, Zhejiang University Lin’an 311300
  • Received:2010-06-30 Revised:2010-09-28 Online:2010-12-25 Published:2010-12-25

摘要:

结构蛋白是细胞壁的重要组成成分。最早发现的细胞壁结构蛋白是伸展蛋白,它在初生壁中约占壁干质量的1%~15%。广义的伸展蛋白是一类富含羟脯氨酸的糖蛋白(HRGP),狭义的伸展蛋白则特指具有Ser-Hyp4重复模体的HRGP分子。以往的研究发现伸展蛋白主要功能是控制细胞的正常伸展,增强细胞壁的机械强度。近年来在拟南芥中进行的伸展蛋白基因缺失突变研究结果显示,伸展蛋白也参与细胞壁的初始形成过程,这是不同于以往对细胞伸展蛋白认识的新发现。伸展蛋白翻译后修饰包括脯氨酸残基羟基化、羟脯氨酸与丝氨酸残基糖基化,在过氧化物酶催化下,形成分子间与分子内交联,通过直链和支链连接进行分子组装。由于伸展蛋白是细胞壁结构蛋白的主要组成成分,研究伸展蛋白的结构和功能对更好地了解细胞壁结构及其组装具有重要意义。

关键词: 伸展蛋白, 交联作用, 糖基化

Abstract:

Structural proteins are important compositions in plant cell wall, and the first structural protein detected in plant cell wall is one of extensins which account for 1%-15% of the dry weight of the primary cell wall.The term extensin generally refers to all hydroxyproline-rich glycoprotein members in an inclusive sense. However, the term is also used to specifically describe HRGPs possessing Ser-Hyp4 repeat motif. Their major functions are to control cell wall extension, and to enhance the mechanical resistance of plant cell wall. In recent years, studies on rsh mutant of Arabidopsis demonstrated that extensins also played an essential role in initiating the cell wall formation, which was a nascent function of extensins. The post-translational modifications of extensins include proline hydroxylation and the subsequent glycosylation of Hyp and Ser residues, intra- and inter-molecular cross-links activated by peroxidase, self-assembly through linear and lateral molecule links. Since extensins are one of the main structural proteins in plant cell wall, studying the function and structure of extensins is important for our better understanding about cell wall structure and cell wall assembly.

Key words: extensin, cross-link, glycosylation

中图分类号: