mtlD/gutD双价基因转化美洲黑杨×青杨的研究

doi:10.11707/j.1001-7488.20020606

林业科学 ›› 2002, Vol. 38 ›› Issue (6): 30-35.doi: 10.11707/j.1001-7488.20020606

mtlD/gutD双价基因转化美洲黑杨×青杨的研究

樊军锋韩一凡李玲彭学贤李嘉瑞

西北农林科技大学林学院,杨凌712100；中国林业科学研究院林业研究所,北京100091；中国科学院微生物研究所,北京100080；西北农林科技大学园艺学院,杨凌712100

收稿日期:2002-07-09 修回日期:1900-01-01 出版日期:2002-11-25 发布日期:2002-11-25

STUDIES ON TRANSFORMATION OF MTLD/GUTD DIVALENT GENES TO POPULUS DELTOIDS×P. CATHAYANA

Fan Junfeng,Han Yifan,Li Ling,Peng Xuexian,Li Jiarui

College of Forestry, Northwest Sci-Tech University of Agriculture and Forestry Yangling712100;Forestry Research Institute, Chinese Academy of Forestry Beijing100091;Institute of Microbiology, Academia Sinica Beijing100080;College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry Yangling712100

Received:2002-07-09 Revised:1900-01-01 Online:2002-11-25 Published:2002-11-25

摘要/Abstract

摘要：

利用叶盘法开展了mtlD/gutD双价基因转化美洲黑杨×青杨的研究,经诱导不定芽及诱导生根阶段卡那霉素(选择性抗生素)连续筛选,获得了38株卡那霉素抗性植株。抗性植株经PCR检测,28株呈阳性。对其中5株PCR阳性植株进行Southern及Western杂交,有4株二者均呈阳性,证明双价基因成功整合到美洲黑杨×青杨基因组中并得到表达。耐盐实验表明这4株转基因植株抗NaCl能力比对照均有不同程度提高。

关键词: 美洲黑杨×, 青杨, mtlD/gutD双价基因, 基因转化, 分子检测, 耐盐性测定

Abstract:

Transformation of mtlD/gutD divalent genes to Populus deltoids × P.cathayana by means of leaf disc was undertaken. Through successive selection in shoot and root induction stage under high level kanamycin pressure, 38 kanamycin-resistant rooted plants were obtained. PCR analysis showed that 28 of these kanamycin-resistant rooted plants were positive. 5 of these 28 PCR positive plants were taken out for Southern blot and Western blot, and the result indicated that 4 of the 5 plants were positive for both. This meant that mtlD/gutD divalent genes were successfully integrated into the genome of these 4 plants, and the proteins expressed by mtlD/gutD divalent genes were synthesized. The salt resistant test demonstrated that salt tolerance ability of these 4 plants was enhanced to some extent.

Key words: Populus deltoids ×, P. cathayana;MtlD/gutD divalent genes;Gene transformation;Molecular biological analysis;Salt resistant test

樊军锋韩一凡李玲彭学贤李嘉瑞. mtlD/gutD双价基因转化美洲黑杨×青杨的研究[J]. 林业科学, 2002, 38(6): 30-35.

Fan Junfeng;Han Yifan;Li Ling;Peng Xuexian;Li Jiarui. STUDIES ON TRANSFORMATION OF MTLD/GUTD DIVALENT GENES TO POPULUS DELTOIDS×P. CATHAYANA[J]. Scientia Silvae Sinicae, 2002, 38(6): 30-35.

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mtlD/gutD双价基因转化美洲黑杨×青杨的研究

STUDIES ON TRANSFORMATION OF MTLD/GUTD DIVALENT GENES TO POPULUS DELTOIDS×P. CATHAYANA

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