关键词: 朱砂叶螨, 萹蓄, 乙酰胆碱酯酶, Na ⁺, K⁺ -ATPase, GSH-S-转移酶

Abstract:

This paper showed a study on acaricidal activities of the plant extracts from Polygonum aviculare against Tetranychus cinnabarinus and the effects on the activities of several relevant enzymes. The plant extracts were extracted using different solvents such as chloroform, petroleum ether and methanol. It was found that the corrected mortality of female adults could reach 89.95% (2 mg·mL^-1) and the half lethal concentration (LC₅₀) was (0.715 3±0.125 3) mg·mL^-1 when using chloroform as the solvent, which exhibited best acaricidal activities among three solvent extracts (P<0.05). Through further extraction and column chromatography, we obtained 19 components from the chloroform extracts, and No.2, 7, 8, 15, 16, 17 and 18 components had acaricidal activities higher than 70%, among them, No.16 caused the mites the highest motality (87.12%). Furthermore, colorimetry assay showed that the component No.16 could affect the activity of acetylcholinesterase (AchE), Na⁺,K⁺ -ATPase, GSH-S-transferase (GSTs) of T. cinnabarinus.

Key words: Tetranychus cinnabarinus, Polygonum aviculare, acetylcholinesterase (AchE), Na⁺, K⁺ -ATPase, GSH-S-transferase