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林业科学 ›› 1998, Vol. 34 ›› Issue (5): 74-78.

• 论文及研究报告 • 上一篇    下一篇

温度周年变化与桑树植原体消长的关系

戴群1,刘秉胜1,何放亭2,陈子文2   

  1. 1. 山东大学生物系 济南 250100
    2. 中国农业科学院植物研究所 北京 100094
  • 收稿日期:1997-10-28 出版日期:1998-09-25 发布日期:1998-09-25
  • 基金资助:
    国家自然科学基金及植物病虫害生物学国家重点实验室开放基金资助项目

RELATIONSHIP BETWEEN THE SEASONAL TEMPERATURE VARIATION AND THE PHYTOPLASMAL AMOUNTS IN MULBERRY TREES

Qun Dai1,Bingsheng Liu1,Fangting He2,Zhiwen Chen2   

  1. 1. Department of Boilogy of Shandong University Jinan 250100
    2. Institute of Plant Protection, CAAS Beijing 100094
  • Received:1997-10-28 Online:1998-09-25 Published:1998-09-25
  • Supported by:
    国家自然科学基金及植物病虫害生物学国家重点实验室开放基金资助项目

摘要:

通过聚合酶链式反应(PCR)检测了植原体在桑树体内的分布及其相对含量的周年变化。结果显示,茎部中植原体的含量在生长季节逐渐升高, 7月达到最大,而冬季检测不到,叶部与茎部相似但植原体的含量在8月达到最大;根部全年均含有植原体,含量较低。结果表明,桑树植原体在寄主体内的消长与温度的周年变化有关。

关键词: 温度, 桑树植原体, 聚合酶链式反应(PCR)

Abstract:

By polymerase chain reaction (PCR), the seasonal variation of the phytoplasmal amounts in mulberry tree was determined.It was demonstrated that the phytoplasma was unevenly distributed in the host.In stem, its amount increased during the growing season, which reached its peak in July and was undetected in winter; in leaves, the situation was similar to that in stem but the peak was in August.In roots, its amount was lower and hardly varied in all the year round.It was indicated that the seasonal variation of phytoplasma in mulberry trees was related to that of temperature.

Key words: Mulberry tree phytoplasma, Polymerase chain reaction(PCR), Temperature