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林业科学 ›› 1996, Vol. 32 ›› Issue (4): 382-384.

• 研究简报 • 上一篇    

筛选与杨树抗云斑天牛基因连锁的RAPD标记*

王京兆1,卞学瑜2,王斌1,韩一凡2   

  1. 1. 中国科学院遗传研究所 北京100101
    2. 中国林业科学研究院林业研究所 北京100091
  • 收稿日期:1995-06-15 出版日期:1996-07-25 发布日期:1996-07-25

SELECTION OF THE RAPD MARKERS LINKED TO INSECT-RESIST GENE(S) IN POPLAR HYBRIDS

Jingzhao Wang1,Xueyu Bian2,Bin Wang1,Yifan Han2   

  1. 1. Institute of Genetics, GAS Beijing 100101
    2. Research Institute of Forestry, CAF Beijing 100091
  • Received:1995-06-15 Online:1996-07-25 Published:1996-07-25

关键词: 杨树, 云斑天牛, RAPD, 多态性产物

Abstract:

DNAs isolated from poplar trees Ⅰ-69(♀)、 Ⅰ-63(♂) and two F1 clones named 34-17 and 34-135 were used as template to detect the polymorphisms by RAPD analysis. Of 200 primers, three primers amplified polymorphic products, and only one of them, OPAD-01 is linked likely to insectresist gene.RAPD analysis of 34-215 (suspective to pests) and 34-188 (resistance to pests) also confirmed the results.

Key words: Batocersa Horsfieldi (Hope.), RAPD, Insect-resistance gene