赤桉人工种子的研制*Ⅰ.幼芽繁殖体的高频率诱导及同步性控制

林业科学 ›› 1996, Vol. 32 ›› Issue (4): 311-316.

赤桉人工种子的研制^*Ⅰ.幼芽繁殖体的高频率诱导及同步性控制

谷瑞升,陈正华,姚渝光,刘桂珍

中国科学院遗传学研究所北京 100101

收稿日期:1995-06-09 出版日期:1996-07-25 发布日期:1996-07-25

MANUFACTURE OF SYNTHETIC SEEDS OF EUCALYPTUS CAMALDULENSIS Ⅰ. THE SYNCHRONOUS AND HIGH FREQUENCY PRODUCTION OF SHOOT TIPS IN VITRO

Ruisheng Gu,Zhenghua Chen,Yuguang Yao,Guizhen Liu

Genetics Institute, Academia Sinica Beijing 100101

Received:1995-06-09 Online:1996-07-25 Published:1996-07-25

摘要/Abstract

摘要：

系统地研究了诱导高频、同步可制成“芽珠”的赤桉幼芽(Shoot tips)的方法, 为以幼芽为繁殖体制备人工种子奠定基础。试验结果:首先, 培养基中适宜的BA和NAA浓度和配比直接控制着制备“芽珠”型幼芽的数量, 同时对已分化出的幼芽进一步伸长有很大的影响, BA为1.0mg/L, NAA为0.5mg/L的培养基中附加40mg/L腺嘌呤和5%的蔗糖能较好地控制幼芽生长及其叶片大小; 其次, 培养过程中先以16天光/暗为10h/14h培养之后转入4天全黑暗培养可控制幼芽生长的同步性, 在半固体培养基上连续继代三次, 获得了每次继代(20天)平均产生25个/cm²适宜制备“芽珠”的幼芽。文中就赤桉培养中褐色渗出物问题进行了初步研究, 通过预培养的方法克服了褐变物对培养中材料的影响。

关键词: 赤桉, 幼芽, 高频率及同步性

Abstract:

This test was carried out with Eucalyptus camaldulensis in vitro to study the high frequency and synchronous production of shoot tips. As 6-Benzylam inopurine (BA) (0.5-1.0mg/L), naphthalene acetic acid (NAA) (0.25-0.5 mg/L) in the medium increasing, more shorter shoots with smaller leaves and more shoot tips suitable for being encapsulated were produced. 40mg/L adenine (ADE) with 1.0mg/L BA and 0.5mg/L NAA in the medium has some role in controleing the shoots and leaves growth, and is fitable for the shoot tip production. The synchronous production of shoot tips was realized through the culture on 16 days light then 4 days dark. In three successive subcultures, mean 25 shoot tips per the material area (cm²) per subculture (20 days) were achieved in the semisolid medium. The problem of brown exudate in shoot subculture was also researched. The measure to overcome was released.

Key words: Eucalyptus camaldulensis, Shoot tips, Synchronous and High frequency

谷瑞升,陈正华,姚渝光,刘桂珍. 赤桉人工种子的研制^*Ⅰ.幼芽繁殖体的高频率诱导及同步性控制[J]. 林业科学, 1996, 32(4): 311-316.

Ruisheng Gu,Zhenghua Chen,Yuguang Yao,Guizhen Liu. MANUFACTURE OF SYNTHETIC SEEDS OF EUCALYPTUS CAMALDULENSIS Ⅰ. THE SYNCHRONOUS AND HIGH FREQUENCY PRODUCTION OF SHOOT TIPS IN VITRO[J]. Scientia Silvae Sinicae, 1996, 32(4): 311-316.

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[2]	谷瑞升,陈正华,关月兰,刘桂珍. 赤桉人工种子制备过程中微芽繁殖体转株的研究[J]. 林业科学, 1998, 34(3): 26-31.