抗虫转基因欧洲黑杨的Western印迹法分析*

林业科学 ›› 1996, Vol. 32 ›› Issue (3): 274-276.

抗虫转基因欧洲黑杨的Western印迹法分析^*

陈颖¹,李强¹,李玲¹,韩一凡¹,田颖川²

1. 中国林业科学研究院林业研究所, 北京 100091
2. 中国科学院微生物研究所, 北京 100080

收稿日期:1994-12-04 出版日期:1996-05-25 发布日期:1996-05-25

WESTERN BLOT ANALYSIS OF TRANSGENIC POPULUS NIGRA PLANTS TRANSFORMED WITH BACILLUS THURINGIENSIS TOXIN GENE

Ying Chen¹,Qiang Li¹,Ling Li¹,Yifan Han¹,Yingchuan Tian²

1. Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091
2. Institute of Microbiology, Academia Sinica Beijing 100080

Received:1994-12-04 Online:1996-05-25 Published:1996-05-25

摘要/Abstract

关键词: Bt.基因, 转基因欧洲黑杨, Western印迹法

Abstract:

Four transgenic Populus nigra plants which were transformed with Bacillus thuringiensis toxin gene and showed high insect resistance were analysed by Western blot method. The result showed that 55kD polypeptides were found in the positive control and four transgenic plants showed Bt. toxin gene was not only inserted into the chromosomes of poplar, but also expressed into protein. The result gave a direct evidence to explain the four plants's high insect resistance.

Key words: Bt.toxin gene, Transgenic Populus nigra, Western blot analysis

陈颖,李强,李玲,韩一凡,田颖川. 抗虫转基因欧洲黑杨的Western印迹法分析^*[J]. 林业科学, 1996, 32(3): 274-276.

Ying Chen,Qiang Li,Ling Li,Yifan Han,Yingchuan Tian. WESTERN BLOT ANALYSIS OF TRANSGENIC POPULUS NIGRA PLANTS TRANSFORMED WITH BACILLUS THURINGIENSIS TOXIN GENE[J]. Scientia Silvae Sinicae, 1996, 32(3): 274-276.

[1]	李玲齐力旺韩一凡汪迎春李文彬. TA29-Barnase基因导致抗虫转基因欧洲黑杨雄性不育的研究[J]. 林业科学, 2000, 36(1): 28-32.
[2]	陈颖,韩一凡,田颖川,李铃,聂绍基. 苏云金杆菌杀虫晶体蛋白基因转化美洲黑杨的研究[J]. 林业科学, 1995, 31(2): 97-102.