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林业科学 ›› 1996, Vol. 32 ›› Issue (3): 243-247.

• 论文及研究报告 • 上一篇    下一篇

苦豆碱对松材线虫的杀线活性*

赵博光   

  1. 南京林业大学, 南京 210037
  • 收稿日期:1994-10-07 出版日期:1996-05-25 发布日期:1996-05-25

NEMATICIDAL ACTIVITY OF ALOPERINE AGAINST PINE WOOD NEMATODES

Boguang Zhao   

  1. Nanjing Forestry University Nanjing 210037
  • Received:1994-10-07 Online:1996-05-25 Published:1996-05-25

摘要:

研究了苦豆草中苦豆碱对松材线虫的杀线活性, 讨论了苦豆草生物碱在研究防治松材线虫病中的意义。使用含苦豆碱的培养基培养灰葡萄抱菌菌丝的生物测定方法, 测定了苦豆碱对松材线虫的杀线活性, 5天后培养基中苦豆碱的LC50=2.63×10-5g/ml, 当浓度为1×10-4g/ml和2.63×10-5g/ml时, 15天后苦豆碱的杀线率分别为99.9%和94.3%。

关键词: 松材线虫病, 松材线虫, 苦豆草, 苦豆碱, 杀线活性

Abstract:

The strong nematicidal activity of aloperine from Sophora alopecuroides L. against pine wood nematode (Bursaphelenchus xylophilus Stener et Buhrer Nickle) is reported for the first time. The nematicidal activity of aloperine against pine wood nematodes was examined by the bioassy in which the medium containing aloperine was used to oulture mycelia of Botrytis cinerea Pers.After 5 days LC= 2. 63 × 10-5g/ml. Moreover, the nematicidal activity of aloperine increased with time. When the levels of aloperine in the bioassy medium were at 1 × 10-4g/ml and 2. 63 × 10-5g/ml, the nematicidal effects after 15 days reached 99. 9% and 94. 3%, respectively. The signifcance of alkaloids from S. alopecuroides in studies on the control of pine wilt desease is discussed.

Key words: pine wilt desease, Bursaphelenchus xylophilus Stener et Buhrer Nickle, Sophora alopecuroides L., Aloperine, Nematicidal effect