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›› 1994, Vol. 30 ›› Issue (5): 385-390.

• 论文及研究报告 •    下一篇

脱落酸诱导黑荆树悬浮细胞抗冻性

王敬文 蒋晶   

  1. 中国林业科学研究院亚热带林业研究所,富阳311400
  • 收稿日期:1993-08-03 修回日期:1900-01-01 出版日期:1994-09-25 发布日期:1994-09-25

INDUCING FREEZING TOLERANCE OF SUSPENSIONCULTURED CELLS IN ACACIA MEARNSII BY ABSCISIC ACID

Wang Jingwen,Jiang Jing   

  1. Research Institute of Subtropical Forestry, CAF Fuyang 311400
  • Received:1993-08-03 Revised:1900-01-01 Online:1994-09-25 Published:1994-09-25

摘要: 研究了脱落酸(ABA)诱导黑荆树悬浮培养细胞抗冻性发育的有关问题,其中包括ABA施用浓度、抗冻性发育的时间进程、ABA诱导可溶性蛋白质含量增高、环己亚胺对抗冻性发育的抑制作用等。研究结果表明,黑荆树悬浮培养细胞能够在添加ABA的MS培养基中,在25℃进行抗寒诱导,以10mg/LABA处理8天的悬浮培养细胞在-6℃的存活率达83.6%,而对照存活率为零。ABA能够诱导悬浮培养细胞可溶性蛋白质含量增高5.5倍,环己亚胺能够抑制蛋白质含量增高,同时也抑制低温驯化和ABA诱导的抗冻性发育。

关键词: 黑荆树, 抗冻性, ABA

Abstract: Role of abscisic acid(ABA) in the induction of freezing tolerance in Acacia mearnsii suspension-cultured cells was studied, which mainly concerned the concentration of exogenous ABA added into medium, the time course for the induction of freezing tolerance,induction of increase in soluble proteins and inhibitory effects of cycloheximide on development of freezing tolerance.The results indicated that Acacia mearnsii suspension-cultured cells could be hardened for 8 days at 25℃ by adding ABA into MS medium. Cells treated with ABA (10mg/L) attained a survival of 83.6% at-6℃, while the survival of the nonhardened(control) was 0.In comparision,the cells treated with ABA increased 5.5 fold more soluble proteins.The biosynthesis of soluble proteins was inhibited by cyclohexomide,and the development of freezing tolerance induced by cold acclimation and ABA was simultaneously prevented.

Key words: Acacia mearnsii, Freezing tolerance, ABA