Abstract: The present paper deals with the karyotype of Cryptomeria fortunei Hooibrenk ex Otto et Dietr. Results indicated that the chromosome of C. fortunei is 2n=22 with ten metacentric (M-type) pairs, one submetac-entric (SM-type) pair. The chromosomes (№g) in the genome showed a long kinetochore region containing several chromosomes, which may be regarded as characteristic of the karyotype of C. fortunei. Results also indicated that the key differential in the karyotype between C. fortunei and C. japonica.