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›› 1984, Vol. 20 ›› Issue (4): 389-396.

• 论文及研究报告 • 上一篇    下一篇

文冠果仁油非皂化物中甾醇部分的分离、含量测定和结构鉴定

严梅和 李佩文 熊丽曾   

  1. 北京师范大学化学系有机化学教研室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1984-11-25 发布日期:1984-11-25

ISOLATION, ESTIMATION AND IDENTIFICATION OF THE STEROLS IN UNSAPONIFIABLE FRACTION OF THE OIL FROM XANTHOCERAS SORBIFOLIA BGE

Yan Meihe,Li Peiwen,Xiong Lizeng   

  1. Department of Chemistry, Beijing Normal University
  • Received:1900-01-01 Revised:1900-01-01 Online:1984-11-25 Published:1984-11-25

摘要: 本文对文冠果仁油非皂化物中的甾醇部分进行了比较全面的分离、含量测定和结构鉴定。采用硝酸银-硅胶G制备薄板、二氯甲烷-石油醚作展开剂,分离效果较好。我们分离和鉴定了α-菠菜甾醇(纯度高于99.5%)、△7-豆甾烯醇(纯度99.5%)、△(5.22)-豆甾醇(纯度98%)和△7-燕麦甾醇(纯度98%),并鉴定了微量的菜油甾醇、β-谷甾醇和胆甾醇。

Abstract: The unsaponifiable matters of the oil from Xanthoceras sorbifolia Bge are separated by TLC with silica gel G into four fractions: sterols, 4-methylsterols, triterpene alcohols and hydrocarbons. The acetates of sterols are separated by TLC with 18% silver nitrate impregnated silica gel G, using CH2CL2-petroleum (7:3 v/v) as developing reagents. The identification of major components is carried out by TLC, m.p.,optical rotation, GLC, IR spectrum and MS or GC-MS.It is found that Δ7-stigmastenol, α-spinasterol and Δ7-avenasterol are present in large amounts in the unsaponifiable fraction, α-spinasterol (purity 99.5%),Δ7-stigmastenol (purity 99.5%) Δ5.22-stigmasterol (purity over 98%) and Δ7-avenasterol (purity 98%) in the sterol fraction are isolated and identified, while trace Δ5-sterols as campesterol, β-sitosterol and cholesterol are identified only.