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林业科学 ›› 2011, Vol. 47 ›› Issue (12): 97-100.doi: 10.11707/j.1001-7488.20111214

• 论文 • 上一篇    下一篇

侧柏枝叶挥发物对小白鼠自发行为的影响

王艳英1, 王成1, 郄光发1, 董建华1, 蒋继宏2   

  1. 1. 中国林业科学研究院林业研究所 国家林业局森林培育重点实验室 国家林业局城市林业研究中心 北京 100091;2. 徐州师范大学 江苏省药用植物生物技术重点实验室 徐州 221116
  • 收稿日期:2010-03-29 修回日期:2010-09-27 出版日期:2011-12-25 发布日期:2011-12-25
  • 通讯作者: 王成

Effect of VOCs from Branch and Leaf of Platycladus orientalis on Locomotor Activity in Mice

Wang Yanying1, Wang Cheng1, Qie Guangfa1, Dong Jianhua1, Jiang Jihong2   

  1. 1. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Centre of Urban Forestry of State Forestry Administration Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091;2. Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province Xuzhou Normal University Xuzhou 221116
  • Received:2010-03-29 Revised:2010-09-27 Online:2011-12-25 Published:2011-12-25

摘要:

采用旷场试验,连续6天观察分析昆明种小白鼠经不同浓度侧柏枝叶挥发气味环境处理后行为指标的变化,研究侧柏枝叶挥发物对小白鼠自发行为的影响,以评价侧柏挥发物的保健功能。结果表明: 1)处理组小白鼠在旷场中的运动总路程大于对照组,且与浓度的大小呈正相关,表明侧柏挥发物有助于提高小白鼠的兴奋性。2)中央格运动总路程、中央格运动时间、站立次数等3项指标呈现阶段性变化,在适应阶段处理组的探究能力大于对照组,熟悉环境阶段则差异不明显,但处理组指标数值的下降幅度远远大于对照组,表明侧柏挥发物在增加小鼠认知能力,提高记忆能力方面起到有利的作用。3)处理组和对照组小白鼠体质量变化相同,但处理组小白鼠的粪便粒数始终比对照组少,说明在侧柏挥发物气体环境中,小白鼠的食欲没有受到影响,精神相对比较轻松。

关键词: 侧柏, 挥发物, 小白鼠, 自发行为, 旷场试验

Abstract:

In order to evaluate the health function of volatile organic compounds (VOCs) from branch and leaf of Platycladus orientalis, effects of the volatile on ICR mice spontaneous behavior were examined for successive 6 days. Using an open-field test the changes of mice behavior indicators were observed under environments with different concentrations of volatile matter. The results were as follows: 1)The total moving distance of treatment group was greater than that of the control group in the open field, and the distance was positively correlated with the concentration of volatile compounds, which demonstrated that P. orientalis volatile stimulated mice. 2) The three indicators, including central grid distance, the central cell exercise time and frequency of standing, presented a stage change. The inquiry ability of treatment group was bigger than the control group at the adaptation stage, however the differences were not significant during the familiar environment stage. Nevertheless, reduction of the indicator values in treatment group was greater than that of the control group, which indicated that P. orientalis volatile played a beneficial role in increasing cognitive and memory abilities of mice. 3)Treatment groups and control group had the same changes in body weight, but the fecal grains of treatment group were always less than the control group, indicating that the P. orientalis volatile odor environment had no influence on mice' appetites, and mice were relatively relaxed with the volatile matter.

Key words: Platycladus orientalis, VOCs, mouse, locomotor activity, open field test

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